Thursday, November 8, 2007

Statistical experimental design was used to optimize the conditions viz. Ethanol, Ammonium phosphate and Biotin concentration of L-Glutamic acid from Brevibacterium roseum NCIM 2238 by submerged fermentation. Maximum L-Glutamic acid concentration of 53.01621 g/l was obtained. The optimum conditions were found to be ethanol concentration of 5.3646 %v/v, ammonium phosphate of 0.7081 %w/v and biotin concentration of 1.6735 μg/ml. Glutamic acid production was also effectively promoted by optimizing the medium composition using Response Surface Methodology (RSM). Basically, this optimization process involves three major steps: performing the statistically designed experiments, estimating the coefficients in the mathematical model, and predicting the response and checking the adequacy of the model. Thus, by using SmF process with Brevibacterium roseum, the Central Composite Design (CCD) was found to be the most favorable strategy for investigating with respect to L-Glutamic acid production. Section #521 Biomems and Microfluidics: Sensing, Detection, and Integration (T3002) Room 355 F (Salt Palace Convention Center) Remarkable progress has been made to fabricate microscale and nanoscale devices for the detection and manipulation of biological cells and molecules. Furthermore, devices with different functions and of different materials have been integrated to form microscale and nanoscale systems for complex procedures. In this session, we invite contributions dealing with any aspects of micro/nanoscale sensors and actuators with biological applications. The interested topics include but are not limited to immunosensors, electrochemical sensors, and various spectroscopic and separation tools in a microchip format. We are particularly interested in papers dealing with micro/nano scale systems and issues related to integration. Both experimental and theoretical contributions are welcome.